CaSki

Description

categories-Reproductive system cancer cell lines-cryovial-organism-Human-tissue-Cervix-disease-Carcinoma-synonyms-Ca-Ski, Ca Ski, Caski, CASKI-age-40 years-gender-Female-ethnicity-Caucasian-morphology-Epithelial-like-cellType-Epidermoid-growth Properties-Adherent-CaSki (CLS catalog number 300145)-biosafety Level-2-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-ACF (CLS catalog number 800650)