Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Embryo-disease–synonyms-C3H/10T1/2 Clone 8, C3H/10T1/2-clone8, C3H/10T1/2 CL8, C3H10T1/2 clone8, C3H10T1/2CL8, 10T1/2(clone8), 10T1/2, C3H10T1-2, C3H10T1/2, C3H-10T1/2, C3H 10T1/2, C3H/10T1/2-age-Embryo-gender–ethnicity–morphology-Fibroblast-cellType–growth Properties-Adherent-C3H/10T1/2, Clone 8 (CLS catalog number 305164)-biosafety Level-1-cultureMedium-BME-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)