Description

categories-Rat cell lines-cryovial-organism-Rat-tissue-Liver-disease–synonyms-BRL3A, BRL 3A, Buffalo Rat Liver-3A-age–gender–ethnicity–morphology–cellType–growth Properties-Adherent-BRL-3A (CLS catalog number 500129)-biosafety Level-1-cultureMedium-Hams F12-mediumSupplements-10% FBS, 1 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)