Description
categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Embryo-disease–synonyms-BALB/c 3T3 clone A31, Balb/c3T3, BALB/c 3T3, Balb/c 3T3, BALB/3T3, Balb/3T3-4-Cl31, 3T3 clone A31, BALB/3T3 cl. A31, BALB 3T3 clone A31, BALB/3T3 (clone A31), B/C3T3, 3T3-A31, 3T3(A31), A31, A31N-age-Embryo, 14 to 17 days gestation-gender–ethnicity–morphology-Fibroblast-cellType–growth Properties-Adherent-BALB/3T3 clone A31 (CLS catalog number 305155)-biosafety Level-2-cultureMedium-DMEM-mediumSupplements-10% FBS-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)
