AT-1

Description

categories-Rat cell lines-cryovial-organism-Rat-tissue-Prostate-disease-Adenocarcinoma-synonyms-R-3327-AT-1, AT1, AT-1-TC, Dunning R-3327 AT-1, R3327-AT1-age–gender–ethnicity–morphology-Epithelial-like-cellType–growth Properties-Adherent. The cells form clusters in soft agar and can be adapted to suspension growth-AT-1 (CLS catalog number 500121)-biosafety Level-1-cultureMedium-RPMI 1640-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)