Description

:AbBy Fluor® 405;host:Rabbit;target_protein:AIF;modification:Unmodified;clonality:Polyclonal;isotype:IgG;concentration:1ug/ul;subcellular_locations:Cytoplasm, Nucleus;source:KLH conjugated synthetic peptide derived from human AIF;gene_id:9131;swiss_prot:O95831;applications_with_dilutions:WB(1:300-5000), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200);cross_reactive_species:Human, Mouse, Rat, Pig;predicted_cross_reactive_species:Dog, Cow, Sheep, Chicken, Rabbit;background:Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces ‘parthanatos’ i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner.;purification:Purified by Protein A.;storage_buffer:Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.;storage_condition:Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.;:401nm/421nm;synonyms:AIF; CMT2D; CMTX4; COWCK; NADMR; NAMSD; PDCD8; COXPD6; Apoptosis-inducing factor 1, mitochondrial; Programmed cell death protein 8; AIFM1