Description

categories-Mouse cell lines-cryovial-organism-Mouse-tissue-Subcutaneous Connective Tissue, Loose Connective Tissue And Fat, Normal-disease–synonyms-A-9, A9 (Hamprecht), A9(Hamprecht), AG 9, GM00346, GM-346, GM346, GM00346B-age-100 days old-gender-Male-ethnicity–morphology-Fibroblast-Like-cellType–growth Properties-Adherent-A9 (CLS catalog number 305166)-biosafety Level-1-cultureMedium-DMEM-mediumSupplements-10% FBS-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)