Description

categories-Prostate cancer cell lines-cryovial-organism-Human-tissue-Prostate-disease-Prostate carcinoma-synonyms-22Rv1, 22Rv-1, 22rV1, CWR-22rv1, CWR22-Rv1, CWR22R-V1, CWR22-R1, CWR22Rv1, CWR22R-age-Adult-gender-Male-ethnicity-European-morphology-Epithelial-cellType–growth Properties-Adherent-22RV1 (CLS catalog number 305037)-biosafety Level-1 (2 when used for genetic engineering)-cultureMedium-RPMI 1640-mediumSupplements-10% FBS-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)