Description

categories-Rat cell lines-cryovial-organism-Rat-tissue-Thyroidea-disease–synonyms-FRT-L, FR-TL, Fischer Rat Thyroid in Low-serum-age-6 weeks-gender-Unspecified-ethnicity–morphology–cellType–growth Properties-Adherent-FRTL (CLS catalog number 500202)-biosafety Level-1-cultureMedium-Coons F12-mediumSupplements-5% FBS-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 5 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)