Description

categories-Lung cancer cell lines-cryovial-organism-Human-tissue-Lung-disease–synonyms-HEL 299, Hel-299, Hel 299, HEL299-age-Fetus-gender-Male-ethnicity-African-morphology–cellType–growth Properties–HEL-299 (CLS catalog number 300193)-biosafety Level-1-cultureMedium-EMEM-mediumSupplements-10% FBS, 2 mM L-glutamine-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300xg, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium. -freezeMedium-CM-1 (CLS catalog number 800150)