|Detection range||31.25-2000 pg/mL|
|Sample type &Sample volume||serum, plasma and other biological fluids; 100μL|
|Specificity||This kit recognizes Rat IL-1β in samples. No significant cross-reactivity or interference between Rat IL-1β and analogues was observed.|
|Reproducibility||Both intra-CV and inter-CV are < 10%.|
|Application||This ELISA kit applies to the in vitro quantitative determination of Rat IL-1β concentrations in serum, plasma and other biological fluids.|
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1β. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IL-1β. You can calculate the concentration of Rat IL-1β in the samples by comparing the OD of the samples to the standard curve.
Kit components & Storage
An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.