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Human primary cells

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categories-Human primary cells-cryovial-organism-Human-tissue-Lung-disease–synonyms-HFL-1, HFL 1, Human fetal lung fibroblast 1, HFL-age-Fetus-gender-Male-ethnicity–morphology-Fibroblast-cellType–growth Properties-Adherent-HFL1 (CLS catalog number 305065)-biosafety Level-1-cultureMedium-Hams F12K-mediumSupplements-10% FBS-subculturing-Remove medium and rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10 ml for T75 cell culture flasks). Add Accutase (1-2 ml per T25, 2.5 ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at ambient temperature for 8-10 minutes. Carefully resuspend the cells with medium (10 ml), centrifuge for 3 min at 300 g, resuspend cells in fresh medium and dispense into new flasks which contain fresh medium.-freezeMedium-CM-1 (CLS catalog number 800150)